REFERENCE:
https://doi.org/10.1101/2020.12.08.415562
https://1drv.ms/b/s!Ath3f5ykGji3mi8xidQEgV1zLX-W?e=FSwVfm
KEY: reconstruction and modeling of embryogenesis
- compare the color-coded fate and quantitative cell morphological properties
- tree-distance to classify asymmetric and symmetric (cell fate)
- hypothesis: contact area-dependent cell communication imposes constraints on embryonic geometries.
- tree-edit distance (time domain, easy to do registration)
Abstract
- invertebrate 無脊椎
- homologous 同源的
- rationale 理由
Introduction
- early embryonic cell lineages are considered invariant
- constitute attractive model systems to study the mechanisms driving cellular reproducibility.
Rationale
- To quantify embryonic reproducibility in the ascidian …, cell lineages, cell shapes, cell interations.
- segmentation and tracking of embryonic cells procedure(ASTEC)
- Development of ascidians is characterized by lower cell number and earlier fate restriction. 94 of 110 early gastrula cells are fate restricted.
Result
- Up to neurula, embryos develop without cell growth , programmed cell death, or cell neghbor exchanges.
- Beyond cell position, cell cycle duration, and cell lineages we observed a high reproducibility of cell arrangements:
- mechanistically, we uncovered a tight link between the control of cell arragements and asymmetric cell divisions.(which give rise to sister cells of distinct fates)
- Combine
Methodology P33
Explanation
- MAXIMUM POINT IN PCA ( SHAPE SPACE) TO DO CLUSTERING
Experiment
- co
ANALYSIS
Conclusion
- Establishes the highly reproducible ascidian embryo as a framework to bridge cell behaviors, morphogenesis, and the underlying regulatory program.
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- segmentation
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- geometric control
